|
| Status |
Public on Apr 09, 2012 |
| Title |
embryo under C2, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Zebrafish embryos exposed to 50 ug/L pentachlorophenol
|
| Organism |
Danio rerio |
| Characteristics |
strain: Tuebingen
genotype/variation: wild type
tissue: whole body
developmental stage: embryos at about 8hpf (gastrula)
treatment: 50 ug/L pentachlorophenol
|
| Treatment protocol |
30 min. egg collections at 25C were exposed in pentachlorophenol solution according to the different concentrations C1-C3. Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were rinsed with PBS and then transferred to a microtube filled with Trizol solution (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (GeneChip Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Zebrafish Genome Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArrayTM scanner 3000.
|
| Description |
Zebrafish_C2-1
Gene expression data from embryos exposed to 50 ug/L pentachlorophenol at 8hpf.
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS) Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The target signal was set to 500.
|
| |
|
| Submission date |
Apr 03, 2012 |
| Last update date |
Jul 29, 2013 |
| Contact name |
Ting Xu |
| E-mail(s) |
[email protected]
|
| Organization name |
Tongji University
|
| Street address |
No. 1239 Road Siping
|
| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE37019 |
Expression data from early zebrafish embryos |
|
