|
| Status |
Public on Sep 12, 2012 |
| Title |
15ADON_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PDR5 mutant strain, 15ADON
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
background/strain: BY4743
genotype/variation: PDR5 knockout mutant
treatment: 15-acetyldeoxynivalenol
|
| Treatment protocol |
The yeast strain was grown in YPD medium with 25 ppm of each type B trichothecene mycotoxin for 2 hours.
|
| Growth protocol |
S. cerevisiae BY4743 (BY4743; MATa/α his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lys2Δ0) derivative pdr5 (PDR5 (YOR153W) knock-out strain) was grown in YPD medium at 25C for 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each sample of total RNA was extracted using the FastRNA Pro Red kit (MP Biomedicals, US) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Approximately 500 ng of total RNA was processed to produce biotinylated aRNA targets using the Affymetrix 3' IVT Express Kit.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures.
|
| Scan protocol |
Standard Affymetrix procedures.
|
| Description |
pdr5_15ADON_2
Gene expression data from PDR5 mutant yeast treated with 15-acetyldeoxynivalenol (15ADON).
|
| Data processing |
Signals were calculated by MAS5.0 using Affymetrix GeneChip Operating Software Version 1.4.
|
| |
|
| Submission date |
Mar 30, 2012 |
| Last update date |
Sep 12, 2012 |
| Contact name |
Tadahiro Suzuki |
| Organization name |
National Food Rsearch Institute
|
| Department |
Applied Microbiology Division
|
| Lab |
Microorganism Evaluation Laboratory
|
| Street address |
kan-nondai 2-1-12
|
| City |
Tukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8642 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE36954 |
Yeast genomic expression patterns in response to type B trichothecenes |
|
