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Sample GSM902184 Query DataSets for GSM902184
Status Public on Mar 27, 2012
Title Lung_SS_biological rep3
Sample type RNA
 
Source name Left lung, in-utero exposure to SHS, adult exposure to SHS
Organism Mus musculus
Characteristics strain: Balb/c
gender: male
age: 15 weeks
tissue: left lung
treatment: in-utero exposure to SHS, adult exposure to SHS
Treatment protocol Animals were exposed to sidestream smoke with a suspended particle load of 10 mg/m3 during the smoke exposure or to filtered-air as control (5 hr/day, 7 days a week).
Growth protocol We housed and handled Balb/c mice on a 12-hr light-dark cycle. Mice had food and water ad libitum except when in the exposure chambers.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions (QIAGEN).
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays in a GeneChip Hybridization Oven 640, followed by washing and staining in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Left lung from 15-week-old male Balb/c mice with in-utero exposure to SHS, adult exposure to SHS.
Data processing The data were analyzed with the R/Bioconductor platform. Packages used: AffyExpress, Limma, Heatplus, etc.
 
Submission date Mar 26, 2012
Last update date Mar 27, 2012
Contact name Rui Xiao
E-mail(s) [email protected]
Organization name Columbia University
Department Anesthesiology
Lab P&S 12-402
Street address 630 West 168th Street
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL1261
Series (1)
GSE36810 Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS)

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
1415670_at 9.537172697
1415671_at 11.24949925
1415672_at 11.68641558
1415673_at 8.677946742
1415674_a_at 9.943328791
1415675_at 9.59088627
1415676_a_at 11.29031768
1415677_at 9.833243985
1415678_at 11.14192192
1415679_at 11.80231904
1415680_at 10.18391157
1415681_at 10.08201165
1415682_at 8.941693725
1415683_at 11.17370898
1415684_at 9.562815759
1415685_at 9.310426323
1415686_at 10.80574168
1415687_a_at 12.83239731
1415688_at 10.79710164
1415689_s_at 9.588133768

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM902184_S6M2SS.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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