|
| Status |
Public on Mar 27, 2012 |
| Title |
Lung_AS_biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Left lung, in-utero exposure to air, adult exposure to SHS
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
gender: male
age: 15 weeks
tissue: left lung
treatment: in-utero exposure to air, adult exposure to SHS
|
| Treatment protocol |
Animals were exposed to sidestream smoke with a suspended particle load of 10 mg/m3 during the smoke exposure or to filtered-air as control (5 hr/day, 7 days a week).
|
| Growth protocol |
We housed and handled Balb/c mice on a 12-hr light-dark cycle. Mice had food and water ad libitum except when in the exposure chambers.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Arrays in a GeneChip Hybridization Oven 640, followed by washing and staining in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Left lung from 15-week-old male Balb/c mice with in-utero exposure to air, adult exposure to SHS.
|
| Data processing |
The data were analyzed with the R/Bioconductor platform. Packages used: AffyExpress, Limma, Heatplus, etc.
|
| |
|
| Submission date |
Mar 26, 2012 |
| Last update date |
Mar 27, 2012 |
| Contact name |
Rui Xiao |
| E-mail(s) |
[email protected]
|
| Organization name |
Columbia University
|
| Department |
Anesthesiology
|
| Lab |
P&S 12-402
|
| Street address |
630 West 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE36810 |
Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS) |
|
