|
| Status |
Public on Jan 01, 2014 |
| Title |
Reh_Ara_HS_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Human leukemia cell line- Reh
|
| Organism |
Homo sapiens |
| Characteristics |
human leukemia cell line: Reh
treatment: Ara-C
stromal cells: HS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using TRI reagent as per manufacturer's instructions. Concentration and Quality of RNA samples were determined with nanodrop 1000 spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared using Illumina TotalPrep RNA amplification kit (Ambion) as per manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Gene expression microarray data was extracted with Bead Studio 3.6 (Illumina, CA, USA). Normalization of data was performed with the quantile normalization method using R 2.9.2 program. Normalized signal intensity values were log 2 base transformed using Gene cluster 3.0 version for further analysis.
|
| |
|
| Submission date |
Mar 08, 2012 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Xiaoping Sun |
| E-mail(s) |
[email protected]
|
| Phone |
7137452000
|
| Fax |
7137924793
|
| Organization name |
M.D. Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE36372 |
Wnt Pathway Contributes to the Protection of Acute Lymphoblastic Leukemia Cells by Bone Marrow Stromal Cells and May Be a Therapeutic Target |
|
