|
| Status |
Public on Feb 21, 2012 |
| Title |
Liver_nash metformin_no.2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, fasting
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
diet: MCD + HF diet mixed with 0.1% metformin (nash metformin)
tissue: liver
|
| Treatment protocol |
Eight-week-old C57BL/6 mice were divided into three experimental groups and fed for 8 weeks as follows: a) normal chow (normal), b) methionine- and choline-deficient diet+ high fat diet (NASH), c) MCD + HF diet mixed with 0.1% metformin (nash metformin).
|
| Growth protocol |
Hepatic tissues were obtained after the sacrifice and immediately frozen in liquid nitrogen and stored at −80°C until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each liver using the RNeasy Mini kit (Qiagen, Tokyo, Japan).
|
| Label |
biotin
|
| Label protocol |
A total of 2 μg of total RNA was labelled according to the small sample labelling protocol recommended by the manufacturer.
|
| |
|
| Hybridization protocol |
After generating the hybridization cocktails, hybridization to the DNA microarray and fluorescent labelling were performed with a GeneChip Hybridization Wash and Stain kit (Affymetrix).
|
| Scan protocol |
The microarrays were subsequently scanned with the GeneChip Scanner 3000 MegAllele System (Affymetrix).
|
| Description |
Gene expression data from mouse liver.
Sample name: Met2
GC1972new.CEL
|
| Data processing |
Data analysis was conducted with the GeneChip Operating System 1.1 (Affymetrix). Detection, signal, signal log ratio, and change were obtained from the GeneChip Operating System (GCOS) with default settings after global normalization was performed to make the average intensity of all probe sets equal to 100.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Feb 21, 2012 |
| Contact name |
Hirofumi Misu |
| E-mail(s) |
[email protected]
|
| Phone |
+81-76-265-2234
|
| Organization name |
Kanazawa University
|
| Street address |
13-1 Takaramachi
|
| City |
Kanazawa |
| ZIP/Postal code |
920-8641 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35961 |
Expression data from mouse liver treated with metformin |
|
