|
| Status |
Public on Mar 19, 2015 |
| Title |
WT Sox9 Vertebral Column Cells-Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
E12.5 Verebral Column
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J;129
tissue: Skeletal
genotype/variation: Sox9 WT
developmental stage: E12.5
|
| Treatment protocol |
Vertebral column was dissected out of WT embryos where Sox9 gene was tagged with EGFP at the 3' UTR vis IRES and homozygous embryos where Sox9 was knocked out by EGFP and subjected to Fluoroscent activated cell sorting
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the sorted cells by Trizol followed by DNAse treatment and QIAGEN RNeasy kit as per manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA samples were prepared using the TargetAmp-Nano labelling kit for Illumina Arrays
|
| |
|
| Hybridization protocol |
Standard Illumina Hyb
|
| Scan protocol |
Standard Illumina Scan
|
| Data processing |
Illumina output was imported to Genome Studio and no normalization was done. Genespring was used to do normalize the data using the following parameters- Normalization Algorithm-Scale. Scale to median of all samples. Threshold raw signal to 1.
|
| |
|
| Submission date |
Feb 17, 2012 |
| Last update date |
Mar 19, 2015 |
| Contact name |
Thomas Lufkin |
| E-mail(s) |
[email protected]
|
| Organization name |
Clarkson University
|
| Department |
Biology
|
| Lab |
Stem Cells & Regenerative Medicine
|
| Street address |
8 Clarkson Avenue
|
| City |
Potsdam |
| State/province |
NY |
| ZIP/Postal code |
13699 |
| Country |
USA |
| |
|
| Platform ID |
GPL6568 |
| Series (2) |
| GSE35877 |
Genome wide binding and expression profiling defines a Bapx1-Sox9 axis in vertebral column, Gut, Spleen and limbs in developing mouse embryo. |
| GSE35898 |
Gene Expression profile of Sox9 sorted cells from Vertebral column of E12.5 wildtype (WT) and Homozygous (HOMO) mouse embryos |
|
