|
| Status |
Public on Aug 01, 2012 |
| Title |
C5_Frontal_Cortex_HIV infected_w/HAD_w/o HIVE |
| Sample type |
RNA |
| |
|
| Source name |
brain_frontal_cortex
|
| Organism |
Homo sapiens |
| Characteristics |
race: White
age: 52
gender: male
phenotype: HIV Infected with neurocognitive impairment (HAD: HIV-associated dementia)
tissue: Frontal cortex
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Human U133 2.0 plus GeneChips were washed and stained.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
41G758.CEL
Gene expression data from the white matter of HIV infected HAD human brain
|
| Data processing |
The data were analyzed with Affy package for R programming.
|
| |
|
| Submission date |
Feb 16, 2012 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Jessica Winkler |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Nebraska Medical Center
|
| Department |
Pharmacology and Experimental Neuroscience
|
| Lab |
Howard Fox
|
| Street address |
985800 Nebraska Medical Center- DRC I
|
| City |
Omaha |
| ZIP/Postal code |
68198 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35864 |
The National NeuroAIDS Tissue Consortium Brain Gene Array: Two types of HIV-associated neurocognitive impairment |
|
