|
| Status |
Public on Feb 08, 2012 |
| Title |
in vitro spleen cells 72hr IM(-)_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
spleen,72hr,IM(-), replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
age: 8 weeks
tissue: splenocytes
agent: control
|
| Treatment protocol |
The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy kit
|
| Label |
Cy3
|
| Label protocol |
After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wash buffer 2 (Agilent)
|
| |
|
| Hybridization protocol |
1650.0 ng at 65 degree_C during 17 hours
|
| Scan protocol |
Arrays were scanned using Agilent G2565AA Microarray Scanner System.
|
| Description |
Gene expression in spleen cells cultured without 10 µM Imatinib in conditioning medium containing IL-4 and LPS for 72 h
US14702391_252665512076_S01_GE1-v5_91_0806_1_3.txt
|
| Data processing |
Data processed with FeatureExrtraction v9.1. For normalization of raw microarray data, 75 percentile shift normalization was applied with GeneSpring GX v11.2.
|
| |
|
| Submission date |
Feb 06, 2012 |
| Last update date |
Feb 08, 2012 |
| Contact name |
toyotaka kawamata |
| E-mail(s) |
[email protected]
|
| Organization name |
The Institute of Medical Science,The University of Tokyo
|
| Department |
Hematology-Oncology/Molecular therapy
|
| Street address |
4-6-1,Shirokanedai,Minato-ku
|
| City |
Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE35559 |
Gene expression profile induced by Imatinib |
|
