|
Status |
Public on Jul 12, 2012 |
Title |
blood-cd1c-dc-4 |
Sample type |
RNA |
|
|
Source name |
healthy donor, dendritic cell, CD1c
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood cell type: dendritic cell cell subtype: CD1c disease state: healthy
|
Treatment protocol |
None.
|
Growth protocol |
Monocytes and dendritic cell subsets were purified by FACS sorting from primary samples for transcription analysis.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from monocytes using an RNeasy Mini Kit (Qiagen) as per manufacturer's specifications. Extracted RNA was runned and quantified on a Bioanalyser (Agilent) for quality control and used subsequently in a microarray assay.
|
Label |
biotin
|
Label protocol |
Total RNA samples were amplified and biotinylated using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX).
|
|
|
Hybridization protocol |
According to BeadChip array manufacturer's protocol.
|
Scan protocol |
According to BeadChip array manufacturer's protocol.
|
Description |
BD_CD1c_DC_III healthy donor, dendritic cell, CD1c,Homo sapiens,blood,dendritic cell,CD1c,total RNA,biotin
|
Data processing |
Array data were extracted at the probeset level with no background correction using Illumina's BeadStudio software. These raw data were normalised using the Loess method within the LUMI package in R/Bioconductor v2.13.1. Resulting p-values were corrected for multiple testing using the Benjamini-Hochberg false discovery rate, and probesets with corrected p-value less than 0.05 were considered.
|
|
|
Submission date |
Jan 31, 2012 |
Last update date |
Oct 23, 2015 |
Contact name |
Pavandip Singh Wasan |
E-mail(s) |
[email protected]
|
Organization name |
Singapore Immunology Network
|
Department |
Bioinformatics
|
Street address |
8A Biomedical Grove, #03-06, Immunos
|
City |
Singapore |
ZIP/Postal code |
138648 |
Country |
Singapore |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE35457 |
Transcriptome profiles of mouse and human monocyte and dendritic cell subsets (human data) |
GSE35459 |
Transcriptome profiles of mouse and human monocyte and dendritic cell subsets |
|
Relations |
Reanalyzed by |
GSE74316 |