|
| Status |
Public on Nov 20, 2024 |
| Title |
Human bronchial epithelial cells, 48hr, MCC, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
BEAS-2B_MCC
|
| Organism |
Homo sapiens |
| Characteristics |
cell: Human bronchial epithelial cells (BEAS-2B)
treatment: MCC
time: 48h
|
| Treatment protocol |
The BEGM was removed, and the cells were treated with working solutions of eight types of CNFs (CNF1, CNF2, CNF3, CNF4, CNF5, CNF6, CNF7, or CNF8) as well as MCC. At this point, the cells were approximately 40% confluent. Then incubated for 48 hours at 37 °C in a humidified incubator with 5% CO₂.
|
| Growth protocol |
Human bronchial epithelial cells (BEAS-2B), obtained from the American Type Culture Collection (ATCC® CRL-9609™), were cultured at 37 °C in 6-well plates using BEGM (Lonza, Basel, Switzerland) in a humidified atmosphere of 95% air and 5% CO₂.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAzol Lysis Reagent (Qiagen, Japan) following the manufacturer’s instructions. RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., US), and sample qualities were monitored with an Agilent 2100 Bioanalyzer (Agilent Technologies, US).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3-labeled cRNA was prepared from RNA using a Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions, followed by RNeasy column purification (Qiagen). Dye incorporation and cRNA yield were checked with the NanoDrop 2000 spectrophotometer.
|
| |
|
| Hybridization protocol |
Each labeled cRNA probe was used separately for hybridization to a 4×44 K Whole Human Genome Microarray (G2519F-014850; Agilent Technologies) at 65 °C for 17 h.
|
| Scan protocol |
Hybridized microarray slides were washed according to the manufacturer’s instructions and scanned with a DNA Microarray Scanner (Agilent) at 5 μm resolution.
|
| Description |
Gene expression after 48hr in MCC-exposed Human bronchial epithelial cells
CF040_09
|
| Data processing |
The scanned images were analyzed numerically using Agilent Feature Extraction Software, version 12.0.3.1.
log2 average normalized signal intensity
|
| |
|
| Submission date |
Nov 15, 2024 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Katsuhide Fujita |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Research Institute of Science for Safety and Sustainability
|
| Street address |
16-1 Onogawa
|
| City |
Tsukuba |
| ZIP/Postal code |
3058569 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE282018 |
Gene expression profiles in human bronchial epithelial cells exposure to cellulose nanofibrils and microcrystalline cellulose |
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