|
| Status |
Public on Nov 21, 2024 |
| Title |
AS-778961-LR-60677 |
| Sample type |
SRA |
| |
|
| Source name |
labyrinth-EC-placenta-rep-1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Placenta
age: E16.5
cell type: Endothelial cells
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole transcriptome scRNA-seq was performed following an adapted SMARTseq2.5 protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
AS-778961-LR-60677
|
| Data processing |
scRNA-sew: Raw sequencing data were demultiplexed and FASTQ files were generated using bcl2fastq software (Illumina, v2.20.0.422). Using salmon, FASTQ files were mapped to the GRCm39 mouse reference genome and count matrices were constructed with the R package tximport.
Assembly: GRCm39
Supplementary files format and content: CSV files containing the count matrix (count_matrix.csv) and the Seurat object metadata (GEO_metadata.csv)
|
| |
|
| Submission date |
Oct 23, 2024 |
| Last update date |
Nov 21, 2024 |
| Contact name |
Stephanie Gehrs |
| Organization name |
DKFZ
|
| Street address |
Im Neuenheimer Feld
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21626 |
| Series (1) |
| GSE280168 |
The spatial zonation of the placental vasculature is specified by epigenetic mechanisms |
|
| Relations |
| BioSample |
SAMN44408175 |
| SRA |
SRX26478357 |
