|
| Status |
Public on Nov 20, 2024 |
| Title |
KJ080_6420 |
| Sample type |
RNA |
| |
|
| Source name |
Constitutive SphK2 KO
|
| Organism |
Mus musculus |
| Characteristics |
biological sex: male
diet: Western diet and sugar water
genotype: SphK2 KO
genetic background: C57BL6-NJ
age (days): 252
diet duration (weeks): 27
|
| Treatment protocol |
diet feeding, ad libitum; see characteristics: Tag_ diet
|
| Growth protocol |
Mice aged 12-18 weeks old were fed diet for 16 weeks prior to sacrifice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizole method
|
| Label |
Nanostring metabolic panel barcode
|
| Label protocol |
RNA target-specific barcoded probes
|
| |
|
| Hybridization protocol |
hybridization of sample to NanoString probes for 16h at 65C in hybridization buffer, according to the manufacturer instructions
|
| Scan protocol |
scanning completed by NanoString nCounter Digital Analyzer
|
| Description |
WDSW-SphK2 KO
|
| Data processing |
Raw counts processed using nSolver Analysis Software; data normalization to internal reference probes and background subtraction performed using Rosalind Software
|
| |
|
| Submission date |
Oct 22, 2024 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Kaitlyn G Jackson |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
VCU
|
| Department |
Immunology
|
| Lab |
Zhou
|
| Street address |
7414 Seminary Ave
|
| City |
Richmond |
| State/province |
Virginia |
| ZIP/Postal code |
23227 |
| Country |
USA |
| |
|
| Platform ID |
GPL35013 |
| Series (1) |
| GSE280083 |
Sphingosine kinase 2 (SphK2) knockout increases liver inflammation in an early MASH mouse model |
|
