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| Status |
Public on Nov 18, 2024 |
| Title |
MLL4+/- Mouse Replicate1 |
| Sample type |
SRA |
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| Source name |
liver
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| Organism |
Mus musculus |
| Characteristics |
tissue: liver
genotype: MLL4+/-
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| Extracted molecule |
total RNA |
| Extraction protocol |
All RNA from liver tissue was isolated using TRIzol (Thermo Fisher Scientific) and RNA samples were treated with DNase (Invitrogen) and then purified using RNeasy mini kit (Qiagen).
RNA-seq libraries were generated according to the TruSeq Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E). RNA-seq libraries were validated by bioanalyzer and real-time RT-PCR.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
5-ML4_MCD1_5788
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| Data processing |
Preprocessing for raw reads was performed to remove low-quality reads and artifacts such as adapter sequences, contaminant DNA, and PCR duplicates.
Assembly: mm10
Supplementary files format and content: xlsx file that contains FPKM values for each sample
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| Submission date |
Sep 19, 2024 |
| Last update date |
Nov 18, 2024 |
| Contact name |
Chong-Su Kim |
| E-mail(s) |
[email protected]
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| Organization name |
Dongduk Women's University
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| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
Gwanak-gu |
| State/province |
--- Select One --- |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE277545 |
The methyltransferase MLL4 promotes non-alcoholic steatohepatitis by enhancing NF-κB signaling |
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| Relations |
| BioSample |
SAMN43826210 |
| SRA |
SRX26124956 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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