|
| Status |
Public on Mar 27, 2012 |
| Title |
siRE_D4_SKNO1_I |
| Sample type |
RNA |
| |
|
| Source name |
RUNX1/ETO knock-down 96h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKNO-1
disease: Acute myeloid leukaemia
time: 96 h after 1st electroporation
|
| Treatment protocol |
SKNO-1 cells were electroporated with 200 nM of either RUNX1/ETO siRNA (siRE) or mismatch siRNA (siMM) in three to four day intervals.
|
| Growth protocol |
SKNO-1 cells were grown in RPMI1640 supplemented with 20% foetal calf serum, 2 mM glutamine and 7 ng/ml GM-CSF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
96 h after 1st electroporation
|
| Data processing |
The data were normalised using quantile normalisation with Lumi R package
|
| |
|
| Submission date |
Dec 20, 2011 |
| Last update date |
May 17, 2012 |
| Contact name |
Salam Adli Assi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Birmingham
|
| Department |
Institute for Cancer and Genomic Sciences
|
| Street address |
IBR
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE29225 |
Depletion of RUNX1/ETO in t(8;21) AML cells leads to genome-wide changes in chromatin structure and transcription factor binding |
| GSE34594 |
Depletion of RUNX1/ETO in t(8;21) AML cells leads to genome-wide changes in chromatin structure and transcription factor binding (Illumina expression) |
|
