|
| Status |
Public on Jan 01, 2012 |
| Title |
esc_het_3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic stem cells, heterozygous
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
cell type: embryonic stem cells (MSCs)
genotype: Mysm1+/tm1a (heterozygous)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were snap-frozen in liquid nitrogen. RNA was isolated using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocol. RNA samples were quantified using NanoDrop™ 1000 (Thermo Scientific), and quality-checked by analysis on an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Illumina TotalPrep-96 RNA Amplification kit (Ambion, UK) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
5183977015_B
het 3
|
| Data processing |
The array probe summaries were calculated in BeadStudio and quantile normalized.
Using limma, a linear model fit was applied and the top differentially expressed genes were tabulated for each contrast using the method of Benjamini and Hochberg to correct the p-values.
|
| |
|
| Submission date |
Dec 08, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Anastasia Nijnik |
| E-mail(s) |
[email protected]
|
| Phone |
15143985567
|
| Fax |
15143982603
|
| Organization name |
McGill University
|
| Department |
Physiology
|
| Street address |
3649 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G0B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE34284 |
Effects of Mysm1 deficiency on gene expression across a range of mouse tissues and cell types (cell data) |
| GSE34285 |
Effects of Mysm1 deficiency on gene expression across a range of mouse tissues and cell types |
|
