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Sample GSM843466 Query DataSets for GSM843466
Status Public on Dec 31, 2013
Title RNA-DHL4-R406-24H-3
Sample type RNA
 
Source name DHL4, R406, 24h
Organism Homo sapiens
Characteristics cell line: DHL4
cell type: diffuse large B-cell lymphoma cells
treatment: R406
treatment duration: 24h
Treatment protocol DLBCL cell lines were grown in RPMI 1640 with 10% fetal calf serum and 2mM glutamine with R406 (1 micromolar) or vehicle (DMSO) alone.
Extracted molecule total RNA
Extraction protocol Cell samples were homogenized in Trizol and RNA was extracted according to standard protocols.
Label biotin
Label protocol cRNA was biotinylated according to the standard Affymetrix protocol.
 
Hybridization protocol According to the manufacturer's protocol.
Scan protocol According to the manufacturer's protocol.
Description DHL4.R406.24h.c
DHL4 DLBCL cell line treated with R406 for 24h.
Data processing The raw .CEL files were converted into probeset-specific expression values using the RMA summarization method as implemented in the BioConductor package 'affy' (Gentleman et al. 2004).
 
Submission date Dec 05, 2011
Last update date Dec 31, 2013
Contact name Stefano Monti
E-mail(s) [email protected]
Organization name Boston University
Department Medicine
Lab Computational Biomedicine
Street address 72 East Concord st. E-613B
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL3921
Series (1)
GSE34176 Gene expression profiling of two diffuse large B-cell lymphoma (DLBCL) cell lines, DHL4 and DHL6, treated with the SYK inhibitor, R406

Data table header descriptions
ID_REF
VALUE RMA-summarized signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 3.2079 A
1053_at 4.0383 A
117_at 3.0269 A
121_at 4.1586 A
1255_g_at 2.9942 A
1294_at 3.0899 A
1316_at 2.833 P
1320_at 2.5773 A
1405_i_at 2.4557 A
1431_at 2.7517 A
1438_at 2.4997 A
1487_at 3.5101 A
1494_f_at 2.9828 A
1598_g_at 3.5394 A
160020_at 3.3358 A
1729_at 6.3433 P
1773_at 2.9672 A
177_at 2.7588 A
179_at 5.7594 A
1861_at 3.1893 P

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM843466.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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