|
Status |
Public on Dec 31, 2013 |
Title |
RNA-DHL4-R406-24H-3 |
Sample type |
RNA |
|
|
Source name |
DHL4, R406, 24h
|
Organism |
Homo sapiens |
Characteristics |
cell line: DHL4 cell type: diffuse large B-cell lymphoma cells treatment: R406 treatment duration: 24h
|
Treatment protocol |
DLBCL cell lines were grown in RPMI 1640 with 10% fetal calf serum and 2mM glutamine with R406 (1 micromolar) or vehicle (DMSO) alone.
|
Extracted molecule |
total RNA |
Extraction protocol |
Cell samples were homogenized in Trizol and RNA was extracted according to standard protocols.
|
Label |
biotin
|
Label protocol |
cRNA was biotinylated according to the standard Affymetrix protocol.
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|
|
Hybridization protocol |
According to the manufacturer's protocol.
|
Scan protocol |
According to the manufacturer's protocol.
|
Description |
DHL4.R406.24h.c DHL4 DLBCL cell line treated with R406 for 24h.
|
Data processing |
The raw .CEL files were converted into probeset-specific expression values using the RMA summarization method as implemented in the BioConductor package 'affy' (Gentleman et al. 2004).
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|
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Submission date |
Dec 05, 2011 |
Last update date |
Dec 31, 2013 |
Contact name |
Stefano Monti |
E-mail(s) |
[email protected]
|
Organization name |
Boston University
|
Department |
Medicine
|
Lab |
Computational Biomedicine
|
Street address |
72 East Concord st. E-613B
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL3921 |
Series (1) |
GSE34176 |
Gene expression profiling of two diffuse large B-cell lymphoma (DLBCL) cell lines, DHL4 and DHL6, treated with the SYK inhibitor, R406 |
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