|
| Status |
Public on Dec 31, 2013 |
| Title |
RNA-DHL4-R406-2H-1 |
| Sample type |
RNA |
| |
|
| Source name |
DHL4, R406, 2h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DHL4
cell type: diffuse large B-cell lymphoma cells
treatment: R406
treatment duration: 2h
|
| Treatment protocol |
DLBCL cell lines were grown in RPMI 1640 with 10% fetal calf serum and 2mM glutamine with R406 (1 micromolar) or vehicle (DMSO) alone.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell samples were homogenized in Trizol and RNA was extracted according to standard protocols.
|
| Label |
biotin
|
| Label protocol |
cRNA was biotinylated according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to the manufacturer's protocol.
|
| Scan protocol |
According to the manufacturer's protocol.
|
| Description |
DHL4.R406.2h.a
DHL4 DLBCL cell line treated with R406 for 2h.
|
| Data processing |
The raw .CEL files were converted into probeset-specific expression values using the RMA summarization method as implemented in the BioConductor package 'affy' (Gentleman et al. 2004).
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Stefano Monti |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston University
|
| Department |
Medicine
|
| Lab |
Computational Biomedicine
|
| Street address |
72 East Concord st. E-613B
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE34176 |
Gene expression profiling of two diffuse large B-cell lymphoma (DLBCL) cell lines, DHL4 and DHL6, treated with the SYK inhibitor, R406 |
|
