|
| Status |
Public on Nov 22, 2012 |
| Title |
ES cells GFP shRNA rep1-rep4 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells GFP shRNA
|
| Organism |
Mus musculus |
| Characteristics |
dev stage: E14
cell type: Embryonic Stem Cells
|
| Treatment protocol |
shRNAs for GFP, Esrrb and Sox2 were transfected into mouse ES cells using lipofecatmine. Transfectants were slected by puromycin for 2 days and RNA harvested for microarray analysis
|
| Growth protocol |
normal ES cell conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRIzol reagent, followed by purification using the QIAGEN RNeasy mini kit according to the manufacturers protocol. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol
|
| Scan protocol |
Standard Illumina Protocol
|
| Data processing |
The data was normalised using GeneSpring 7. Merged 4 biological replicates to give a mean (transformed) expression and stdev value.
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Nov 22, 2012 |
| Contact name |
Andrew P Hutchins |
| E-mail(s) |
[email protected]
|
| Organization name |
Southern University of Science and Technology
|
| Department |
Bioinformatics
|
| Lab |
Bioinformatics and Genomics
|
| Street address |
1088 Xueyuan Rd
|
| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE34170 |
Dual knockdown of Esrrb and Sox2 in mouse embryonic stem cells |
|
