|
| Status |
Public on Dec 03, 2013 |
| Title |
BALB/c intestine FD MTHFR+/- biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Intestine tissues from BALB/c mice with mild MTHFR deficiency fed with low folate diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
tissue: duodenum
genotype: MTHFR+/-
treatment: folate deficient diet
gender: female
|
| Treatment protocol |
Mice were sacrificed and intestines were removed to examine for tumour incidence under dissecting microscope. Normal intestines were frozen at -80 degree.
|
| Growth protocol |
After weaning, BALB/c Mthfr +/- mice and C57Bl/6 Mthfr +/- mice were fed with a control diet (CD, 2mg folate/kg) and a low folate diet (FD, 0.3mg folate/kg) for 1 year. Both diets contain succinylsulfanthiozole (1%) to prevent folate synthesis by intestine microbial biota.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
liquid nitrogen extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeled and fragmented ssDNAs were prepared according to the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay from 100ng of Total RNA (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay User guide P/N 701880 Rev. 5)
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 1.856ug of ssDNA were hybridized for 17 hrs at 45C on GeneChip® Mouse Gene 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The arrays were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from dissected duodenum tissue
|
| Data processing |
The data were analyzed with FlexArray using Affymetrix default analysis settings and APT as normalization method.
|
| |
|
| Submission date |
Nov 29, 2011 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Daniel Leclerc |
| E-mail(s) |
[email protected]
|
| Phone |
514-412-4400 x23281
|
| Fax |
514-412-4331
|
| Organization name |
Montreal Children's Hospital
|
| Department |
Medical Genetics, Room 242
|
| Lab |
Rima Rozen Laboratory
|
| Street address |
4060 Ste-Catherine West
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3Z 2Z3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE34011 |
Expression data from mouse intestine: BALB/c MTHFR+/+ on control diet vs BALB/c MTHFR+/- on folate deficient diet |
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