|
| Status |
Public on Nov 19, 2011 |
| Title |
Wholebrain_NOD_Cloz_DD675_1.1 |
| Sample type |
RNA |
| |
|
| Source name |
brain,Wholebrain,NOD,Cloz
|
| Organism |
Mus musculus |
| Characteristics |
strain: NOD
treatment: clozapine
part of brain: Wholebrain
body wt (g): 28.9
dob: 2009-08-30
weaned: 2013-09-24
litter size: 11
mouse id: DD0675
time inject: 12:30
time sac: 3:30
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen brains using a Biopulverizer (Biospec). Pulverized samples were placed in tubes containing 1ml Trizol reagent (Invitrogen) and a stainless steel bead. Samples were shaken for 5 minutes on a Tissuelyzer apparatus (Mixer Mill 300, Qiagen) and incubated at room temperature for 5 minutes. We added 200 µl chloroform (Fisher) and then samples were sequentially shaken for 15 seconds on a Tissuelyzer, incubated at room temperature for 3 minutes, and centrifuged at 10,000 rpm for 15 min at 4°C. The supernatant was transferred to a gDNA Eliminator spin column (RNeasy plus mini kit, Qiagen) and processed according to the manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
All samples were processed with the GeneChip WT terminal labeling and hybridization protocol in conjunction with the Ambion WT expression kit according the manufacturers instructions.
|
| |
|
| Hybridization protocol |
All samples were processed with the GeneChip WT terminal labeling and hybridization protocol in conjunction with the Ambion WT expression kit according the manufacturers instructions.
|
| Scan protocol |
Affymetrics GeneChip
|
| Data processing |
Affymetrics Expression Console V1.1
|
| |
|
| Submission date |
Nov 18, 2011 |
| Last update date |
Nov 19, 2011 |
| Contact name |
Vasyl Zhabotynsky |
| E-mail(s) |
[email protected]
|
| Phone |
(919) 843-6475
|
| Fax |
(919) 843-4682
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Street address |
120 Mason Farm Road 5000 D, Genetic Medicine Building
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE33822 |
Transcriptome Atlases Of Mouse Brain Reveals Differential Expression Across Brain Regions And Genetic Backgrounds |
|
