|
| Status |
Public on Jan 01, 2022 |
| Title |
Lmajor_KO_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Leishmania major infected miR-223 KO mice replicate2
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
Sex: male
tissue: bone marrow cells
genotype/variation: miR-223 KO
|
| Treatment protocol |
Mice were subcutaneously inoculated with Leishmania major promastigotes at right hind foot pad for 4 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the miRNeasy Kit (Qiagen, 217004) following the manufacturer's recommendations. The protocol includes on-column DNase digestion. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared using Low Input Quick Amp Labeling Kit, One-Color (Agilent, 5190-2305) according to manufacturer's instructions (One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling, v6.5, May 2010), followed by RNAeasy column purification (Qiagen, 4106).
|
| |
|
| Hybridization protocol |
Hybridization and washing were done using Agilent Gene Expression Hybridization Kit (Agilent, 5188-5242) and Agilent Wash Buffer Kit (Agilent, 5188-5327) according to manufacturer's protocols (One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling, v6.5 May 2010).
|
| Scan protocol |
Slides were scanned after washing on the Agilent Microarray Scanner (G2565CA) using one color scan setting for 4x44k array slides (Profile: AgilentHD_GX1col, Resolution: 5 μm).
|
| Description |
Mac1+ cells were sorted from bone marrow cells and analyzed.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Further data processing was done using GeneSpring GX Software v11.0.
|
| |
|
| Submission date |
Nov 17, 2011 |
| Last update date |
Jan 01, 2022 |
| Contact name |
Yoko Fukuda Yuzawa |
| Organization name |
Max-Planck Institute of Immunobiology
|
| Street address |
Stübeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE33765 |
Leishmania major infected WT and miR-223 KO mice |
|
