Male C57Bl/6 Mice were 10.5 weeks old at time of sacrifice.
Treatment protocol
Male C57Bl/6 mice injected i.p. 2x/week for 4 weeks with 50mg/kg miR-122 ASO, sacrificed 2 days after last dose.
Extracted molecule
total RNA
Extraction protocol
For RNA isolation, liver was homogenized in RNeasy RLT buffer using Polytron homogenizer, layered onto 5.7M CsCl2 and pelleted after overnight centrifugation at 35000 rpm, room temp. The pellet was resuspended in RLT buffer and RNA was isolated on RNeasy midi columns following the manufacturer's instructions, and including an on-column DNase digestion step.
Label
biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
mir-122 antisense used is a 2'-O-methoxyethyl phosphorothioate modified antisense RNA oligonucleotide with sequence 5'-ACAAACACCAUUGUCACACUCCA-3'
