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Status
Public on Mar 01, 2006
Title
Saline T00278945
Sample type
RNA
Source name
liver
Organism
Mus musculus Characteristics
Male C57Bl/6 Mice were 10.5 weeks old at time of sacrifice.
Treatment protocol
Mice injected i.p. 2x/week for 4 weeks with saline, sacrificed 2 days after last dose.
Extracted molecule
total RNA
Extraction protocol
For RNA isolation, liver was homogenized in RNeasy RLT buffer using Polytron homogenizer, layered onto 5.7M CsCl2 and pelleted after overnight centrifugation at 35000 rpm, room temp. The pellet was resuspended in RLT buffer and RNA was isolated on RNeasy midi columns following the manufacturer's instructions, and including an on-column DNase digestion step.
Label
biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
Control group
Data processing
CodeLink Expression Analysis 4.1.0.29054
Submission date
Nov 11, 2005
Last update date
Nov 14, 2005
Contact name
Michael Pear
E-mail(s)
[email protected] Phone
760-603-2779
URL
http://www.isspharm.com Organization name
Isis Pharmaceuticals, Inc.
Department
Research Informatics
Street address
1896 Rutherford Rd
City
Carlsbad
State/province
CA
ZIP/Postal code
92009
Country
USA
Platform ID
GPL2897 Series (1)
GSE3603 Microarray analysis of in vivo inhibition of mir-122 with an antisense oligonucleotide.
Data table header descriptions ID_REF VALUE Intensity normalized by median of spot-based raw intensity
RAW_INTENSITY Raw signal intensities corrected for background
QUALITY Numerical Quality score from codes G,S (Good, Saturated) =>1, L (near background) => .5, I, C, CI, CL (Ireggular, Background Contaminated) => 0
Data table ID_REF VALUE RAW_INTENSITY QUALITY 1001
65.57011987
1447.226074
1002
2.226913672
49.15116119
1
1003
0.218191076
4.815788269
0.5
1004
1.004832444
22.17808533
1
1005
0.536941581
11.85106659
0.5
1006
0.451701484
9.969696045
0.5
1007
87.35872152
1928.131592
1008
86.37319604
1906.379639
1010
1.010755818
22.30882263
1
1012
0.159877855
3.5287323
0.5
1013
0.846475876
18.68292999
1
1014
92.22227031
2035.477051
1015
95.11122848
2099.240479
1017
0.133537577
2.947364807
0.5
1018
0.178564688
3.941177368
0.5
1019
0.298784353
6.594596863
0.5
1020
0.181229791
4
0.5
1021
98.89159079
2182.678467
1022
68.75440606
1517.507813
1024
0.135363052
2.98765564
0.5
36227 1149 Kbytes .Supplementary data files not provided
