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Sample GSM829768 Query DataSets for GSM829768
Status Public on Jan 20, 2014
Title HFD 619, fat mRNA
Sample type RNA
 
Source name fat from female offspring of fathers fed on high-fat diet
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
gender: female
tissue: fat
treatment: father fed high-fat diet
Treatment protocol Fathers fed on HFD vs. control were mated with females on normal chow. The female offspring's fat transcriptomics were analyzed through microarray analysis.
Growth protocol At 15 weeks of age, fathers were mated with normal females consuming chow to generate the F1 offspring.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Rat Gene ST 1.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix Scanner GC3000.
Description Gene expression of fat from female offspring of fathers fed high-fat chow.
Data processing RMA normalization was carried out using Partek Genome Suite v6.4.
 
Submission date Nov 08, 2011
Last update date Jan 20, 2014
Contact name Margaret J Morris
E-mail(s) [email protected]
Phone +61293851560
Organization name University of New South Wales
Department School of Medical Sciences
Lab Head of Pharmacology
Street address University of New South Wales
City Wallace Wurth Building, Sydney
State/province NSW
ZIP/Postal code 2052
Country Australia
 
Platform ID GPL6247
Series (2)
GSE33551 Effects of dietary obesity in fathers on gene expression of fat in the female offspring (mRNA data)
GSE33564 Effects of dietary obesity in fathers on gene expression of fat in the female offspring

Data table header descriptions
ID_REF
VALUE RMA signal intensity in log2 space

Data table
ID_REF VALUE
10700001 12.3863
10700002 7.28074
10700003 10.51
10700004 5.27191
10700005 9.23452
10700006 3.50834
10700007 3.71491
10700008 3.32849
10700009 8.69793
10700010 3.9532
10700011 5.26944
10700012 4.74344
10700013 11.7378
10700014 10.5634
10700015 8.85124
10700016 3.50461
10700017 6.66169
10700018 3.39552
10700019 3.59327
10700020 12.4533

Total number of rows: 29214

Table truncated, full table size 481 Kbytes.




Supplementary file Size Download File type/resource
GSM829768.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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