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Status |
Public on Jan 20, 2014 |
Title |
HFD 619, fat mRNA |
Sample type |
RNA |
|
|
Source name |
fat from female offspring of fathers fed on high-fat diet
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague Dawley gender: female tissue: fat treatment: father fed high-fat diet
|
Treatment protocol |
Fathers fed on HFD vs. control were mated with females on normal chow. The female offspring's fat transcriptomics were analyzed through microarray analysis.
|
Growth protocol |
At 15 weeks of age, fathers were mated with normal females consuming chow to generate the F1 offspring.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Rat Gene ST 1.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Scanner GC3000.
|
Description |
Gene expression of fat from female offspring of fathers fed high-fat chow.
|
Data processing |
RMA normalization was carried out using Partek Genome Suite v6.4.
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|
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Submission date |
Nov 08, 2011 |
Last update date |
Jan 20, 2014 |
Contact name |
Margaret J Morris |
E-mail(s) |
[email protected]
|
Phone |
+61293851560
|
Organization name |
University of New South Wales
|
Department |
School of Medical Sciences
|
Lab |
Head of Pharmacology
|
Street address |
University of New South Wales
|
City |
Wallace Wurth Building, Sydney |
State/province |
NSW |
ZIP/Postal code |
2052 |
Country |
Australia |
|
|
Platform ID |
GPL6247 |
Series (2) |
GSE33551 |
Effects of dietary obesity in fathers on gene expression of fat in the female offspring (mRNA data) |
GSE33564 |
Effects of dietary obesity in fathers on gene expression of fat in the female offspring |
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