|
| Status |
Public on Jun 18, 2024 |
| Title |
SREBP-Gal4 control,RNA-seq,Rep2 [w2] |
| Sample type |
SRA |
| |
|
| Source name |
3rd instar larval fat body
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: 3rd instar larval fat body
genotype: SREBP-Gal4/+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by standard protocol with Trizol; 1ug RNA was used for librariy construction
RNA-seq library was prepared by using Illumina TruSeq Stranded Total RNA kit, CUT&Run library was prepared by using illumina TruSeq ChIP library preparation kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Description |
SREBP-Gal4 control 2
axn slmb RNAi count table
axni_slmbi_cts.txt
|
| Data processing |
RNA-Star alignment to dm6 reference with default setting
Gathering counts with FeatureCount with default setting
DESeq2 to normalize counts and generate differential gene expression table with p.adj < 0.05 filter
CUT&Run reads went through nf-core/cutandrun pipeline for trimming, alignment and deduplication
MACS3 for peak calling and normalization
Assembly: dm6
Supplementary files format and content: tab delimited table, count table
Supplementary files format and content: bigwig file, normalized CUT&Run reads
|
| |
|
| Submission date |
Apr 18, 2024 |
| Last update date |
Jun 18, 2024 |
| Contact name |
Jun-yuan Ji |
| E-mail(s) |
[email protected]
|
| Organization name |
Tulane University
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Street address |
1700 Tulane Avenue
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
|
| Platform ID |
GPL32218 |
| Series (1) |
| GSE264356 |
Wnt/Wingless signaling promotes lipid mobilization through signal-induced transcriptional repression |
|
| Relations |
| BioSample |
SAMN41010365 |
| SRA |
SRX24304938 |
