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Sample GSM8066620 Query DataSets for GSM8066620
Status Public on Aug 27, 2024
Title NOD_4F [IgG]
Sample type protein
 
Source name Serum
Organism Mus musculus
Characteristics isotype: IgG
gender: Female
genotype: NOD.B10
age (month): 6
slide: 1
block: 4
Extracted molecule protein
Extraction protocol Serum samples from recipients collected as per routine protocol as defined above were procured from histocompatibility lab after IRB approval. Sera of all subjects were aliquoted and stored at -80ºC.
Label cy3
Label protocol 8 positive controls (4 mouse IgG/IgM controls with different dilutions and 4 human IgG /IgM control with different dilutions) were also imprinted on the arrays as positive controls.
Mouse serum samples were first treated with DNAse I to remove free-DNA and then applied onto antoantigen arrays with 1:50 dilution. The autoantibodies binding to the antigens on the array were detected with cy3-labeled anti-human IgG and the array slides were scanned with Genepix 4400A scanner with laser wavelengths 532nm for cy3 and 635nm for cy5 to generate Tiff images. Genepix Pro 7.0 software is used to analyze the image and generate the Genepix report (GPR) files (Molecular Devices, Sunnyvale, California, USA). The net fluorescent intensity (NFI) of each antigen was generated by subtracting the local background and negative control (Phosphate buffered saline or simplified as PBS) signal.
 
Hybridization protocol not provided
Scan protocol not provided
Description Slide1_532.gpr
Data processing NFI were normalized by robust linear model using build-in Ig control with various dilutions
 
Submission date Feb 06, 2024
Last update date Aug 27, 2024
Contact name Chengsong Zhu
E-mail(s) [email protected]
Phone 2146455160
Organization name UT Southwestern Medical Center
Department Immunology
Lab Microarray & Immune Phenotyping Core
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platform ID GPL34168
Series (1)
GSE255215 Tlr7 mediates tissue-specific disease manifestations in primary Sjogren’s disease in a sex-biased manner

Supplementary data files not provided

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