|
| Status |
Public on Jan 18, 2024 |
| Title |
NPC-24h-rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Neural precursor cell culture
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Adult hippocampus
timepoint: Proliferating
hours differentiating: 0
|
| Treatment protocol |
Cells were harvested at different times after withdrawal of bFGF and EGF to induce differentiation
|
| Growth protocol |
Proliferating cells were maintained on poly-D-lysine/laminin-coated plates in Neurobasal media supplemented with 2 % B27, GlutaMAX, 20 ng/ml bFGF and 20 ng/ml EGF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed on the culture dish and total RNA was prepared using the Qiagen RNeasy micro kit
|
| Label |
biotin
|
| Label protocol |
Amplification and biotin labeling with the TotalPrep™ RNA Amplification Kit (Illumina)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were log2 transformed and normalized using quantile normalization with beadarray (version 2.46.0) in R/BioConductor. Probes were assigned to Entrez GeneIDs using the illuminaMousev1p1.db package (version 1.26.0). Unmapped genes were removed and replicate expression data for the same GeneID were also removed, retaining only the gene with the highest mean.
|
| |
|
| Submission date |
Jan 15, 2024 |
| Last update date |
Jan 18, 2024 |
| Contact name |
Rupert W Overall |
| Organization name |
Humboldt University of Berlin
|
| Street address |
Philippstraße 13
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6105 |
| Series (2) |
| GSE253304 |
Activating transcription factors Atf3/Atf4 drive early molecular events preceding neural stem cell differentiation and control neuron formation [Long] |
| GSE253534 |
Activating transcription factors Atf3/Atf4 drive early molecular events preceding neural stem cell differentiation and control neuron formation |
|
