|
| Status |
Public on Sep 26, 2011 |
| Title |
Cohyb_H1_D0 vs D90_Methy_A |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
genomic DNA extracted from undifferentiated hESCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: embryonic stem cell
cell line: H1
differentian stage: D0
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted by standard phenol-chloroform isolation
|
| Label |
cy3
|
| Label protocol |
2µg of the digested and amplified DNA was labelled from each sample with either Cy3 or Cy5 according to the Agilent Chip-on-chip mammalian protocol (version 9.1, 2006)
|
| |
|
| Channel 2 |
| Source name |
genomic DNA extracted from hESCs with 90 days after undirected differentiation
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: embryonic stem cell
cell line: H1
differentian stage: D90
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted by standard phenol-chloroform isolation
|
| Label |
cy5
|
| Label protocol |
2µg of the digested and amplified DNA was labelled from each sample with either Cy3 or Cy5 according to the Agilent Chip-on-chip mammalian protocol (version 9.1, 2006)
|
| |
|
| |
| Hybridization protocol |
Hybridization of the arrays was carried out according to the Agilent Chip-on-chip mammalian protocol (version 9.1, 2006)
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505B scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
|
| Data processing |
Agilent Feature Extraction Software (9.1.3.1) was used for background subtraction and LOWESS normalization. Probe ratios within one SmaI interval were averaged and the median of the P value log10 ratios of the probes within one SmaI interval was calculated.
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Sep 26, 2011 |
| Contact name |
Robert A Waterland |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Street address |
1100 Bates St.,
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL14617 |
| Series (1) |
| GSE32371 |
DNA methylation and gene expression changes during human embryonic stem cell differentiation |
|
