|
| Status |
Public on Apr 30, 2012 |
| Title |
iKras5-C-on |
| Sample type |
RNA |
| |
|
| Source name |
parental culture of iKras5, on dox
|
| Organism |
Mus musculus |
| Characteristics |
genotype: p48Cre tetO_LKrasG12D ROSA_rtTAL+ p53L+
background strain: FVB/B6 mix
cell type: parental culture of iKras5
treatment: doxycycline
|
| Treatment protocol |
On doxycycline.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by RNeasy column cleanup was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Parental culture of iKras5.
|
| Data processing |
Briefly, raw probe intensities were normalized across arrays with quantile normalization using the R package developed by B. Bolstad. The software package LIMMA (Linear Models for Microarray Data) (Smyth, 2004) was applied to detect significantly differentially expressed probes using Benjamini-Hochberg adjusted P-values.
|
| |
|
| Submission date |
Sep 21, 2011 |
| Last update date |
Apr 30, 2012 |
| Contact name |
Sujun Hua |
| E-mail(s) |
[email protected]
|
| Organization name |
MD Anderson Cancer Center
|
| Lab |
Ronald DePinho Lab
|
| Street address |
1901 East Rd
|
| City |
Houston |
| State/province |
Tx |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32277 |
Kras is required for pancreatic tumor maintenance through regulation of hexosamine biosynthesis and the non-oxidative pentose phosphate pathway |
|
