|
| Status |
Public on Apr 05, 2024 |
| Title |
Peripheral blood cells, EPO, 4.0 hour, biol rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
Blood
|
| Organism |
Xenopus tropicalis |
| Characteristics |
tissue: Blood
cell line: Total peripheral blood cells
cell type: Blood cells
genotype: Wild type
treatment: Culture with EPO
|
| Treatment protocol |
5,000,000 cells were suspended in 7/9 x IMDM-10% FCS and reacted with 1 ng/mL Xenopus tropicalis EPO expressed in E.coli for 1.5 or 4.0 hours.
|
| Growth protocol |
Blood cells were corrected from the heart and mixed with anti-coagulator EDTA-2Na. Cells were washed three times with 7/9 x phosphate buffered serine containing 2 mM EDTA-2Na and 2% fetal calf serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by the acid guanidine phenol-chloroform method. 50 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-Seq were prepared by Lasy-Seq v1.1 (https://sites.google.com/view/lasy-seq/)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
113
|
| Data processing |
Read 1 reads were processed with fastp (version 0.21.0) 1 using the following parameters: --trim_poly_x -w 20 --adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT -l 31.
The trimmed reads were then mapped to the reference transcriptome sequences of X. tropicalis was prepared from the Xenbase v10.0 (https://www.xenbase.org/xenbase/), using BWA mem (version 0.7.17-r1188) 2 with the default parameters.
The read count for each gene was calculated with salmon using -l IU, which specifies the library type (version v0.12.0) 3.
Then, using R (version 4.3.2) 4, the sums of read counts per gene were calculated.
Assembly: UCB_Xtro_10.0
Supplementary files format and content: csv data file includes raw counts for each sample.
|
| |
|
| Submission date |
Dec 22, 2023 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Kazuki Omata |
| E-mail(s) |
[email protected]
|
| Organization name |
Waseda University
|
| Department |
Graduate school of advanced science and engineering
|
| Street address |
Wakamatsucho, 2-2
|
| City |
Shinjuku |
| State/province |
Tokyo |
| ZIP/Postal code |
332-0031 |
| Country |
Japan |
| |
|
| Platform ID |
GPL30213 |
| Series (1) |
| GSE251901 |
Effect of erythropoietin on the gene expression of Xenopus tropicalis peripheral blood cells. |
|
| Relations |
| BioSample |
SAMN39083346 |
| SRA |
SRX23007707 |
