|
| Status |
Public on Oct 31, 2011 |
| Title |
Wild Type B220+, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild Type B220+
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
genotype/variation: wild type
age: 4-6wks
tissue source: bone marrrow or spleen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
B cells were obtained from bone marrow or spleen by MACS using bead conjugated to antibodies against B220 or IgM. RNA was prepared using the RNeasy Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
cRNA was synthesized using the One-Cycle Target Labeling and Control Reagent package (Affymetrix, Inc.).
|
| |
|
| Hybridization protocol |
Fragmentation/hybridization to MOE430A arrays (Affymetrix) were performed according to Affymetrix protocols.
|
| Scan protocol |
Imaging was performed according to standard Affymetrix protocol.
|
| Description |
Gene expression data from EμΜyc mice 4-6 weeks old.
|
| Data processing |
The data were analyzed using GeneSpring GX version 11 based on GCRMA analysis default settings.
|
| |
|
| Submission date |
Sep 20, 2011 |
| Last update date |
Oct 31, 2011 |
| Contact name |
John Lamoine Cleveland |
| Organization name |
H. Lee Moffitt Cancer Center
|
| Street address |
12902 USF Magnolia Drive
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE32239 |
Gene Expression profiling of Myc-induced lymphomagenesis |
|
