|
| Status |
Public on Nov 10, 2011 |
| Title |
Untreated XEN cells rep2 |
| Sample type |
RNA |
| |
|
| Source name |
IM8A1 XEN cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: PO strain
treatment group: untreated
|
| Treatment protocol |
XEN cells were treated with BMP2 (20 ng/ml), activin A (30 ng/ml), both or niether for 6 days.
|
| Growth protocol |
IM8A1 XEN cells were grown in GMEM+10%FBS at 37oC, 5%CO2 in a humidified environment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with QIAGEN's RNeasy Midi kit, and cDNA was prepared using Affymetrix one-cycle kit.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
EukGE-WS2v5
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000
|
| Description |
Gene expression data of untreated XEN cells
|
| Data processing |
GCOS 1.4; Algorithm: ExpressionStat 5.0
|
| |
|
| Submission date |
Sep 18, 2011 |
| Last update date |
Nov 10, 2011 |
| Contact name |
Tilo Kunath |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0) 131 651 9500
|
| Organization name |
University of Edinburgh
|
| Department |
Centre for Regenerative Medicine
|
| Street address |
5 LIttle France Drive
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32199 |
BMP and Activin treatment of mouse extraembryonic endoderm (XEN) cells |
|
