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Status
Public on Mar 14, 2012
Title
uvmo-2_rep4
Sample type
RNA
Source name
CD4 T cell clone
Organism
Mus musculus Characteristics
cd4 t cell clone: uvmo-2
Treatment protocol
harvested at 72 hours
Growth protocol
Usual growth medium (RPMI 1640; 10^-5M 2 mercaptoethanol; 1x glutamax-1 (gibco); recombinant IL-1a, IL-2, IL-6, IL-7, IL-15, TNF-a, IFN-4a, IFN-b)
Extracted molecule
total RNA
Extraction protocol
RNAeasy with DNAse1 treatment step (Qiagen)
Label
biotin
Label protocol
RNA was labeled using Affymetrix standard WT protocol (non-Ambion) using 100ng total RNA.
Hybridization protocol
Hybridization was done using Affymetrix standard hybridization protocol for Gene ST 1.0 arrays
Scan protocol
Scanned using GCOS
Data processing
Partek was used to generate RMA.RMA signals were generated for the core probe sets using the RMA background correction, Quantile normalization and summarization by Median Polish. Summarized signals for each probe set were log2 transformed.
Submission date
Sep 14, 2011
Last update date
Mar 14, 2012
Contact name
Raymond Morris Johnson
E-mail(s)
[email protected] Organization name
Yale University
Department
Med/Infectious Diseases
Street address
PO Box 208022
City
New Haven
State/province
CT
ZIP/Postal code
06520-8022
Country
USA
Platform ID
GPL6246 Series (1)
GSE32128 Plac8-dependent and iNOS-dependent T cell-mediate mechanisms clear Chlamydia muridarum infections from the genital tract
Data table header descriptions ID_REF VALUE RMA log base 2 transformed
Data table ID_REF VALUE 10344614
6.05661
10344616
2.62535
10344618
2.76748
10344620
3.45039
10344622
6.87172
10344624
10.2057
10344633
10.2719
10344637
7.71445
10344653
3.68861
10344658
8.59891
10344674
3.08985
10344679
5.04688
10344705
4.99139
10344707
9.59614
10344713
10.2559
10344715
3.67313
10344717
4.73002
10344719
5.70722
10344721
2.5223
10344723
7.5838
28853 475 Kbytes .Supplementary file Size Download File type/resource GSM796428.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
