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Sample GSM792594 Query DataSets for GSM792594
Status Public on Sep 09, 2011
Title Rad53_3
Sample type genomic
 
Channel 1
Source name rad53Δ, sml1 Δ
Organism Saccharomyces cerevisiae
Characteristics genotype/variation: rad53Δ, sml1 Δ
cell cycle phase: G1 phase
Growth protocol Strains were grown 12-16 hr at 30˚C in YPD. The strains were diluted to O.D 0.1 in YPD and harvested after 5 hr at logaritmic phase .
Extracted molecule genomic DNA
Extraction protocol DNA from FACS-sorted cells was extracted using the MasterPure Yeast DNA purification kit
Label Cy5
Label protocol DNA was digested with DpnII and amplified by four rounds of random-octamer-primed Exo-Klenow DNA synthesis (Invitrogen BioPrime ArrayCGH amplification kit) with amino-allyl modified nucleotides. DNA was labeled with Cy dyes
 
Channel 2
Source name rad53Δ, sml1 Δ
Organism Saccharomyces cerevisiae
Characteristics genotype/variation: rad53Δ, sml1 Δ
cell cycle phase: S phase
Growth protocol Strains were grown 12-16 hr at 30˚C in YPD. The strains were diluted to O.D 0.1 in YPD and harvested after 5 hr at logaritmic phase .
Extracted molecule genomic DNA
Extraction protocol DNA from FACS-sorted cells was extracted using the MasterPure Yeast DNA purification kit
Label Cy3
Label protocol DNA was digested with DpnII and amplified by four rounds of random-octamer-primed Exo-Klenow DNA synthesis (Invitrogen BioPrime ArrayCGH amplification kit) with amino-allyl modified nucleotides. DNA was labeled with Cy dyes
 
 
Hybridization protocol Agilent yeast ChIP-on-chip44Kx4 arrays
Scan protocol Agilent microarray scanner
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
Data processing Matlab was used to read the files, background subtraction and LOWESS normalization.
 
Submission date Sep 08, 2011
Last update date Sep 09, 2011
Contact name Ariel Gispan
E-mail(s) [email protected]
Organization name Weizmann Institute of Science
Department Molecular Genetics
Lab Barkai
Street address P.O. Box 26
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platform ID GPL10930
Series (1)
GSE32002 Replication Timing in Yeast: CGH microarrays: S vs G1 cells in various stress conditions

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (S phase cells /G1 or G2 cells) representing test/reference.

Data table
ID_REF VALUE
A_75_P01000003 null
A_75_P01000016 1.38E+00
A_75_P01000071 -7.81E-01
A_75_P01000148 -4.64E-01
A_75_P01000219 6.62E-02
A_75_P01000274 1.42E+00
A_75_P01000289 -4.20E-01
A_75_P01000310 5.79E-01
A_75_P01000338 1.58E-01
A_75_P01000360 1.75E-01
A_75_P01000376 -3.81E-01
A_75_P01000394 1.27E+00
A_75_P01000410 4.16E-02
A_75_P01000426 2.05E-01
A_75_P01000446 4.20E-01
A_75_P01000473 -6.69E-01
A_75_P01000507 -1.93E+00
A_75_P01000523 -4.21E-01
A_75_P01000549 -3.39E-01
A_75_P01000563 -6.38E-01

Total number of rows: 41480

Table truncated, full table size 993 Kbytes.




Supplementary file Size Download File type/resource
GSM792594_WIS_251481011119_S01_CGH_107_Sep09_1_2.txt.gz 13.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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