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Sample GSM787632 Query DataSets for GSM787632
Status Public on Apr 08, 2012
Title Drosophila melanogaster adult male rep1
Sample type RNA
 
Source name adult male
Organism Drosophila melanogaster
Characteristics strain: Oregon R
Sex: male
developmental stage: adult
tissue: whole organism
Growth protocol Flies were grown at 22C on cornmeal media. Male and female larva were separately flash frozen on dry ice and stored at -80C. Virgin male and female newly eclosed flies were collected, aged for 3-5 days at 22C, and then frozen at -80C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the samples using TRIzol (Invitrogen Inc, Carlsbad, CA), following the manufacter's instructions.
Label Cy3
Label protocol 1000ng of total RNA in 10ul of RNase-free water was used to generate double-stranded cDNA using the Amino Allyl MessageAmp II aRNA Amplification kit (Ambion, Inc., Austin, TX), following the manufacter's instructions. The cDNA was then coupled to Cy3 reactive dye using the same kit, followed by a column purification using the kit-supplied equipment and reagents. Dye incorporation and cDNA yield were checked on a NanoDrop Spectrophotometer.
 
Hybridization protocol 825ng of Cy3 labelled cDNA was fragmented and hybridized to the Agilent-018972 D. melanogaster Oligo Microarray 4x44K G2519F (GPL6385), following the manufacturer's instructions. After 17 hrs of hybridization at 65C, arrays were washed using the Agilent wash buffers per the manufacturer's instructions.
Scan protocol Slides were scanned on the Axon 4000B scanner using the Agilent HD_GX_1Color setting (scan area 61x21.5 mm, scan resolution 5 um, and Green PMT set to XDR hi 100% and XDR low 10%).
Description Biological replicate 1 of 1
Data processing Scanned images were analyzed with the Agilent Feature Extraction Software version 10.5.1.1 (protocol GE1_105_DEC08) to obtain background substracted and spatially detrended processed signal intensities.
 
Submission date Aug 29, 2011
Last update date Apr 08, 2012
Contact name Richard Meisel
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77204-5001
Country USA
 
Platform ID GPL6385
Series (1)
GSE31722 Sex-biased gene expression in Drosophila melanogaster larvae

Data table header descriptions
ID_REF
VALUE Signal intensities for each probe were background corrected and normalized across arrays using default method of the LIMMA package in the R Statistical Computing Environment.

Data table
ID_REF VALUE
1 51114.32465
2 30.79534136
3 22.59060046
4 26.62662393
5 26.62662393
6 24.57679488
7 29.73637589
8 31.16691295
9 30.79534136
10 33.6542131
11 34.02627994
12 9694.449655
13 591.5746548
14 21904.0434
15 252.1996548
16 212151.9809
17 446.8871548
18 15277.82465
19 212151.9809
20 94.63770214

Total number of rows: 45020

Table truncated, full table size 778 Kbytes.




Supplementary file Size Download File type/resource
GSM787632_201006041043_S01_Green_REAL_GE1_105_Dec08_1_4.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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