|
| Status |
Public on Oct 28, 2024 |
| Title |
miR-195_Ebf1–/– Lin–_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
miR-195 virus infected Ebf1–/– Lin– cells, replicate1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Fetal liver Lin– cells
developmental stage: early B cell (around pro B cell)
|
| Treatment protocol |
cultured with each 10ng/mL SCF, IL-7 and Flt-ligand
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the Rneasy(Qiagen) following the manufacturer's recommendations.
|
| Label |
Cy3
|
| Label protocol |
100 ng RNA were labeled with Cyanine-3 (Cy3) using Agilent Whole Mouse Genome Microarray 4x44K v2 (Agilent) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
600 ng of Cy3-labelled cRNA were hybridized using Gene Expression Hybridization Kit according to the manufacturer's instructions.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner ( G2600D SG13164306) using one color scan setting for 4x44k array slides using scan protocol AgilentHD_HiSen_GX_1Color.
|
| Description |
Gene expression of miR-195 transduced Ebf1–/– Lin– cells after B cell developing stimulation
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 11.5.1.1 (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 026655_D_F_20110614) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Oct 31, 2023 |
| Last update date |
Oct 28, 2024 |
| Contact name |
Ryo Yanagiya |
| E-mail(s) |
[email protected]
|
| Phone |
81-6-6879-8294
|
| Organization name |
Research Institute for Microbial Diseases, Osaka University
|
| Department |
Department of Regulation of Infectious Cancer
|
| Street address |
3-1 Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE246669 |
Analysis of expression profiles in miR-195 transduced Ebf1–/– Lin– cells |
|
