|
| Status |
Public on Oct 17, 2023 |
| Title |
Mixed spleen and lymph node naiveCD4 T cells (RNA), freshly isolated, untreated, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
mixed spleen and lymph node
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mixed spleen and lymph node
cell type: NaiveCD4 T cell
genotype: wildtype C57BL/6 Foxp3-GFP polyclonal
treatment: none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy Kit
Takara SMARTer Stranded Total RNA-seq Kit v2 Pico Input Mammalian
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Hisat2/featureCounts
bigwigCompare/deepTools (3.5.1.0.1)
GRCm38/mm10
tab-delineated text matrix includes raw counts for each sample (n = 12) and 54,532 features
bigwigCompare output (bigWig) file that represents the "sum" of reads in all sample replicates with binSize=50
|
| |
|
| Submission date |
Sep 28, 2023 |
| Last update date |
Oct 17, 2023 |
| Contact name |
Daniel L Mueller |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Minnesota Medical School
|
| Department |
Medicine
|
| Lab |
WMBB 3-186
|
| Street address |
2101 6th Street SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE244278 |
BATF represses BIM to sustain tolerant T cells in the periphery (RNA-Seq) |
|
| Relations |
| SRA |
SRX21870500 |
| BioSample |
SAMN37525934 |
