|
| Status |
Public on Aug 29, 2012 |
| Title |
Favorable Histology Wilms Tumor (WT00-081) |
| Sample type |
RNA |
| |
|
| Source name |
Pretreatment tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Stage: IV
age: 85m
relapse: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol (Invitrogen, Carlsbad, CA) extraction and treated with DNase I (Roche, Indianapolis, IN).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The labeled, fragmented cRNA was hybridized to the Affymetrix HG - U133A oligonucleotide array for 16 hours at 45°C according to Affymetrix protocol. Staining was done in the GeneChip Fluidics Station using pre-programmed Affymetrix protocols.
|
| Scan protocol |
The arrays were scanned in an HP GeneArray Scanner.
|
| Data processing |
Data were normalized using robust multi-array average (RMA) method. The normalized value of each probeset for each tumor is provided in the attached excel spreadsheet All Normalized Data.
|
| |
|
| Submission date |
Aug 16, 2011 |
| Last update date |
Aug 29, 2012 |
| Contact name |
Elizaebth J Perlman |
| E-mail(s) |
[email protected]
|
| Phone |
773-880-4306
|
| Fax |
773-880-3858
|
| Organization name |
Northwestern University
|
| Department |
Pathology
|
| Street address |
2373 North Lincoln Avenue
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60614 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE31403 |
Clinically Relevant Subsets Identified by Gene Expression Patterns Support a Revised Ontogenic Model of Wilms Tumor: A Children’s Oncology Group Study |
|
