|
| Status |
Public on May 03, 2012 |
| Title |
PDGF-DD_treated_SMCs |
| Sample type |
RNA |
| |
|
| Source name |
Rat aortic smooth muscle cells treated with PDGF-DD (30 ng/mL)
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
|
| Treatment protocol |
Cells were treated with IL-1b (2.5 ng/mL; R&D Systems) or vehicle (0.1% bovine serum albumin [BSA] and 10 mM acetic acid), or PDGF-DD (30 ng/mL; Zymogenetics) or vehicle (0.01% BSA) for 24 hours.
|
| Growth protocol |
Rat aortic SMCs were grown to confluency in serum-containing DMEM/F12 media for 3 days, then serum starved in serum-free media for 3 days, and then were treated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
10 ug of cRNA was hybridized for 16 hours on Affymetrix rat 230 2.0 GeneChips and stained using Affymetrix Fluidics Station 450 according to Affymetrix protocols.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
Gene expression data from PDGF-DD-treated rat aortic smooth muscle cells
|
| Data processing |
Array normalization and processing was performed using the ExpressionFileCreator module of GenePattern (Broad Institute). MAS5 was used for processing, and normalization was performed with median scaling. Significant differences were defined as fold changes greater or less than or equal to 2 and signal differences in signal intensity greater than or equal to 100.
|
| |
|
| Submission date |
Aug 01, 2011 |
| Last update date |
May 03, 2012 |
| Contact name |
Gary Owens |
| Organization name |
University of Virginia
|
| Department |
Molecular Physiology and Biological Physics
|
| Lab |
Gary Owens' Laboratory
|
| Street address |
415 Lane Road
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE31080 |
Effect of interleukin-1 and PDGF-DD on SMCs |
|
