|
Status |
Public on Oct 05, 2005 |
Title |
M-CSF macrophage without stimulation/CD14 PMNC slide B, first trial |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
M-CSF macrophage without stimulation
|
Organism |
Homo sapiens |
Characteristics |
Culture of CD14 cells with M-CSF for 7 days.
|
Extracted molecule |
total RNA |
Label |
Cy3
|
|
|
Channel 2 |
Source name |
CD14 PMNC
|
Organism |
Homo sapiens |
Characteristics |
CD14 cells were seperated from PBMN by magnetic sorting.
|
Extracted molecule |
total RNA |
Label |
Cy5
|
|
|
|
Description |
RNA amplified before cDNA synthesis.
|
Data processing |
Lowess normalization (GeneSpring software)
|
|
|
Submission date |
Oct 04, 2005 |
Last update date |
Jul 27, 2006 |
Contact name |
Vahid Khajoee |
E-mail(s) |
[email protected]
|
Organization name |
Kyuhsu University
|
Department |
Pediatrics
|
Street address |
3-1-1 Maidashi, higashi-ku
|
City |
Fukuoka |
State/province |
Fukuoka |
ZIP/Postal code |
812-8582 |
Country |
Japan |
|
|
Platform ID |
GPL1294 |
Series (1) |
GSE3408 |
Novel Roles of Osteopontin and CXC Chemokine Ligand 7 in the Defense against Mycobacterial Infection |
|
Data table header descriptions |
ID_REF |
|
VALUE |
same as UNF_VALUE but with flagged values removed |
Spot labels |
Physical place of spots on slide B. |
ARM Dens - ALU / mm2-CH1 |
Artifact-removed density value for each spot for Cy3 signal (M-CSF macrophage without stimulation). |
Bkgd-CH1 |
Background density or volume for Cy3 signal (M-CSF macrophage without stimulation). |
sARMDens-CH1 |
ARM Density value of the spot, minus the background Density value for Cy3 signal (M-CSF macrophage without stimulation). |
S/N-CH1 |
Spot density minus Background density, divided by the SD of the Background density for Cy3 signal. |
Flag-CH1 |
The value is 0 when there is no dust and 1 when dust can be seen for spots from Cy3 signal. |
ARM Dens - ALU / mm2-CH2 |
Artifact-removed density value for each spot for Cy5 signal (CD14 PMNC). |
Bkgd-CH2 |
Background density or volume for Cy5 signal (CD14 PMNC). |
sARMDens-CH2 |
RM Density value of the spot, minus the background Density value for Cy5 signal (CD14 PMNC). |
S/N-CH2 |
Spot density minus Background density, divided by the SD of the Background density for Cy5 signal. |
Flag-CH2 |
The value is 0 when there is no dust and 1 when dust can be seen for spots from Cy5 signal. |
UNF_VALUE |
Log ratio of normalized data for slide B (M-CSF macrophage without stimulation/CD14 PMNC). |