For expansion (de-differentiation), cells were trypsinized and seeded at 3.2X10^4/cm2 in ultra-low attachment plates (Corning). SFM consisted of CMRL 1066 containing 5.6 mM D-glucose and supplemented with 1% BSA fraction V (Sigma), ITS (Gibco-Invitrogen), penicillin (50 units/ml), and streptomycin (50 µg/ml).
Extracted molecule
total RNA
Extraction protocol
according to the manufacturer's protocol (Trizol, Sigma-Aldrich)
Label
biotin
Label protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Hybridization protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Description
gene expression data of de-differentiated cells
Data processing
Gene-level analyses was performed. RMA normalization, summarization by mean and batch effect removal, all were done by Partek Genomics suite (version 6.5) Copyright.
