|
| Status |
Public on Dec 01, 2011 |
| Title |
MDMs with IFN at T2 |
| Sample type |
RNA |
| |
|
| Source name |
IFNα2 pre-treated monocytes-derived macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: monocytes-derived-macrophages
ifn pre-treatment: yes
hiv infection: none
time: 2 hours
|
| Treatment protocol |
Purified primary macrophages were treated with 1000 IU/ml of IFNα2, for 18 hours before infection. Pre-treated macrophages were infected with the Bal strain of HIV-1 at an MOI of 1
|
| Growth protocol |
Monocytes were plated into 25cc flasks in RPMI 1640 supplemented with 10% human serum and incubated at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated for each condition and time point using the TRIzol method according to the manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA were converted to cDNA, which was amplified and transcribed to produce biotinylated cRNA using the Two-Cycle cDNA synthesis kit (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
15ug of fragmented cRNA were hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA) for 16 h at 45°C with constant rotation at 60 rpm. The arrays were washed and stained using the Affymetrix Fluidics Station 450 (protocol EukGE-WS2v5_450).
|
| Scan protocol |
Arrays were read using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from IFNα2 pre-treated macrophages at 2hours
MS_2_IFN
|
| Data processing |
The data were analyzed with GC Robust Multi-array Average (GCRMA) for normalization
|
| |
|
| Submission date |
Jul 10, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Frederic Raymond |
| Organization name |
Université Laval
|
| Department |
École de nutrition
|
| Lab |
INAF
|
| Street address |
2440 Bd Hochelaga Suite 1710
|
| City |
Quebec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE30536 |
Expression data from IFN alpha 2-treated macrophages infected with HIV |
|
