GEO help: Mouse over screen elements for information.
Status
Public on Nov 01, 2005
Title
Col-0 6h MOCK replicate 1
Sample type
RNA
Source name
lateral root inducible segment (between root meristem and hypocotyl)
Organism
Arabidopsis thaliana
Characteristics
Wild type, root segments of 3 day old seedlings, NPA grown plus 6h mock treatment
Extracted molecule
total RNA
Extraction protocol
Root segments as described in Himanen et al (2002; Plant Cell, 14, 2339-2351) were dissected from 3 day old seedlings, germinated on 10 µM NPA and an additional 6h mock-treatment. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 5.8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type third instar larvae imaginal wing discs
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Sep 21, 2005
Last update date
Aug 28, 2018
Contact name
Steffen Vanneste
E-mail(s)
[email protected]
Phone
+32 (0) 93313935
Fax
+32 (0) 93313809
URL
http://www.psb.ugent.be/
Organization name
VIB-University of Ghent
Department
Plant Systems Biology
Lab
Root Development
Street address
Technologiepark 927
City
Ghent
ZIP/Postal code
9052
Country
Belgium
Platform ID
GPL198
Series (1)
GSE3350
SLR/IAA14-dependent auxin induced lateral root initiation
Relations
Reanalyzed by
GSE118579
Reanalyzed by
GSE119083
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
244901_at
122.2
P
0.023926
244902_at
127.1
P
0.030273
244903_at
208.8
P
0.005859
244904_at
24.3
A
0.601074
244905_at
89.4
A
0.149658
244906_at
427.3
M
0.056152
244907_at
9.1
A
0.80542
244908_at
9.9
A
0.943848
244909_at
113.4
A
0.129639
244910_s_at
94.2
P
0.023926
244911_at
4.9
A
0.633789
244912_at
3458.8
P
0.001221
244913_at
121.3
A
0.72583
244914_at
36.7
A
0.432373
244915_s_at
121.6
A
0.303711
244916_at
62
A
0.466064
244917_at
4
A
0.981445
244918_at
111.3
A
0.19458
244919_at
115
A
0.171387
244920_s_at
3041
P
0.000732
Total number of rows: 22810 Table truncated, full table size 596 Kbytes .Supplementary file
Size
Download
File type/resource
GSM75511.CEL.gz
3.3 Mb
(ftp) (http)
CEL