|
| Status |
Public on Sep 29, 2011 |
| Title |
vret homozygous mutant ovaries, repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
fly ovaries, vret homozygous mutant
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: vret homozygous
tissue: ovary
sample type: mutant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Qiagen RNeasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
100 ng total RNA was converted to biotinylated cRNA using the Affymetrix 3' IVT Express kit.
|
| |
|
| Hybridization protocol |
10 micrograms of biotin-labeled cRNA were hybridized for 16 hrs onto the GeneChip Drosophila Genome 2.0 Arrays (Affymetrix).
|
| Scan protocol |
Affymetrix 7G GeneArray scanner.
|
| Description |
vret148-60_vret148-60_R2
Transposable elements expression data from vret homozygous mutant ovaries.
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX11 (Agilent) software. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Jul 01, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
[email protected]
|
| Phone |
+33648065733
|
| Organization name |
IARC WHO
|
| Street address |
150 COURS ALBERT THOMAS
|
| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE30360 |
Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila |
|
| Relations |
| Reanalyzed by |
GSE119084 |
