|
| Status |
Public on Feb 12, 2024 |
| Title |
bl6_intestine_S2_exp1 [HT_MG-430_PM] |
| Sample type |
RNA |
| |
|
| Source name |
Intestine, Bl6 Mice healthy 2 weeks post BAMLET treatment by oral gavage
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
mouse strain: C57BL/6J
tissue: Intestine
experiment: 2w
treatment: BAMLET
|
| Treatment protocol |
Ten-week-old female C57BL/6 mice received 20 mg of BAMLET in 2 x 200 μl PBS (BAMLET) or 2 x 200 μl of PBS alone by daily gavage for ten days. The mice were sacrificed two or five weeks after the end of treatment. Alternatively, mice received 20 mg of BAMLET in 5 ml PBS or PBS alone, daily in the drinking water until sacrifice after eight weeks. Healthy untreated C57BL/6 mice intestines were used as controls.
|
| Growth protocol |
C57BL/6 mice were obtained from Jackson Laboratories at approximately eight weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At sacrifice, bladders were aseptically harvested for mRNA extraction and analysis. Total RNA was extracted (RNeasy Mini kit, Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA was amplified, labeled and fragmented using the GeneChip 3´IVT PLUS Kit ( ThermoFisher Scientific). Biotinylated cRNA was prepared according to the standard Affymetrix's protocol.
|
| |
|
| Hybridization protocol |
6ug of fragmented and labelled cRNA was hybridized onto Mouse Genome 430 PM array strips (Affymetrix) for 16 hours at 45 °C, washed, stained (Applied Biosystems, ThermoFisher Scientific).
|
| Scan protocol |
Scanning was done using the GeneAtlas systems (Thermo Fisher).
|
| Description |
Gene expression data from healthy C57BL/6 mice
|
| Data processing |
Transcriptomic data was normalized using Robust Multi Average implemented in the Transcriptome Analysis Console software (v.4.0.1.36, Applied Biosystems, ThermoFisher Scientific).
|
| |
|
| Submission date |
Jun 26, 2023 |
| Last update date |
Feb 12, 2024 |
| Contact name |
Ines Ambite |
| E-mail(s) |
[email protected]
|
| Organization name |
Lund University
|
| Department |
Laboratory Medicine
|
| Lab |
MIG
|
| Street address |
Klinikgatan 28
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE235765 |
Expression data from healthy C57BL/6 mice treated with BAMLET |
| GSE235767 |
Colon cancer treatment with BAMLET |
|
