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Sample GSM749317 Query DataSets for GSM749317
Status Public on Dec 13, 2011
Title R11_782_Pipes7
Sample type RNA
 
Source name Tumor cells alone
Organism Mus musculus
Characteristics background strain: C57BL/6
cell line: Ep5 (EpRas) cells
platelet description: none
treatment protocol: Sub-confluent EP cells alone
Growth protocol Ep5 cells cultured in DMEM 10% FCS, 1% Penicillin/Streptomycin and 2 mM L-Glutamine
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen RNAeasy
Label biotin
Label protocol Nugen ApplauseĀ® WT-Amp Plus ST System
 
Hybridization protocol Performed according to manufacturer's instructions (Affymetrix) for Affymetrix Mouse Gene 1.0 ST arrays.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G; GeneChipĀ® Command Console.
Data processing Data processing was done using Partek Genomics Suite 6.5 software. RMA was used to summarize and quantile normalize and the probe data into probe set expression values using the adjust for GC content and background correction options.
 
Submission date Jun 27, 2011
Last update date Dec 13, 2011
Contact name Charles Arthur Whittaker
E-mail(s) [email protected]
Organization name Koch Institute
Street address 77 Mass Ave 76-189
City Cambridge
State/province MA
ZIP/Postal code 02152
Country USA
 
Platform ID GPL6246
Series (1)
GSE27456 Direct Signaling Between Platelets and Cancer Cells Induces an Epithelial-Mesenchymal-Like Transition and Promotes Metastasis

Data table header descriptions
ID_REF
VALUE Gene expression values in log2 space from Partek Genomics Suite 6.5.

Data table
ID_REF VALUE
10338001 12.2421
10338002 4.82621
10338003 10.428
10338004 9.12215
10338005 5.7284
10338006 4.38872
10338007 4.1423
10338008 4.14749
10338009 6.88231
10338010 5.65318
10338011 4.59538
10338012 4.77141
10338013 7.00887
10338014 6.77554
10338015 7.08773
10338016 6.31487
10338017 13.182
10338018 5.28671
10338019 4.36222
10338020 6.96217

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM749317_R11_782_Pipes7.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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