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Sample GSM745924 Query DataSets for GSM745924
Status Public on Jun 22, 2011
Title Activated ko biological replicate 1
Sample type RNA
 
Source name CD8+CD28low regulatory T cells
Organism Mus musculus
Characteristics strain: C57Bl/6
genotype: AIRE ko
treatment: Cells cultured 96h with allogenic APC and anti CD3 antibody
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol from Invitrogen for lysis of cells and phase separation according to the manufacturers instructions. RNA was then purified from the aqueous phase using the Qiagen RNeasy Mini Kit. The protocol for RNA clean up was used according to the manufacturer’s instructions.
Label Biotin
Label protocol Total RNA was converted to biotinylated sense DNA as per the Affymetrix Genechip Whole Transcript (WT) Sense Target Labeling Assay Manual.
 
Hybridization protocol Genechips were hybridised overnight at 45 degrees C for 17 hours in an Affymetrix Hybridisation oven.
Scan protocol Genechips were scanned using the Affymetrix GSC3000 scanner.
Description Activated_2_KO
Gene Expression Data
Data processing Data processed with Partek Genomics Suite using RMA background correction and quantile array normalisation.
 
Submission date Jun 21, 2011
Last update date Jun 22, 2011
Contact name Mark B Van der Hoek
E-mail(s) [email protected]
Organization name University of Adelaide
Street address North Terrace
City Adelaide
State/province South Australia
ZIP/Postal code 5005
Country Australia
 
Platform ID GPL6246
Series (1)
GSE30129 AIRE-deficient CD8+CD28low regulatory T lymphocytes fail to control experimental colitis

Data table header descriptions
ID_REF
VALUE log2 RMA corrected intensity

Data table
ID_REF VALUE
10338001 11.5889
10338002 5.19873
10338003 9.46792
10338004 8.64856
10338005 5.42975
10338006 4.80386
10338007 4.53483
10338008 4.47787
10338009 7.12054
10338010 5.55575
10338011 4.83739
10338012 5.03786
10338013 7.44756
10338014 6.71804
10338015 7.25173
10338016 6.21409
10338017 13.1193
10338018 5.28446
10338019 4.72057
10338020 6.89862

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM745924.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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