|
| Status |
Public on Dec 31, 2011 |
| Title |
3x Ez f/wt |
| Sample type |
RNA |
| |
|
| Source name |
adult hearts
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed background of C57BL/6
genotype: Ezh2f/f
tissue: heart
developmental stage: adult
|
| Treatment protocol |
The right ventricle and the interventricular septum of five wild type (Ezh2f/f) and four Ezh2-deficient (Ezh2f/f;Mef2cAHF::Cre) mice were isolated in cold PBS and homogenized in Trizol reagent (Invitrogen).
|
| Growth protocol |
Dissected adult hearts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix methods for biotin labeling were used.
|
| |
|
| Hybridization protocol |
Affymetrix mouse Gene ST 1.0 arrays were hybridized according to manufacturer recommendations.
|
| Scan protocol |
Affymetrix mouse Gene ST 1.0 arrays were scanned according to manufacturer recommendations.
|
| Description |
wild type
|
| Data processing |
Raw intensities from CEL files were analyzed using Affymetrix Power Tools (APT, version 1.10.1) to generate RMA (robust multi-array average) expression intensities on a log2 scale for each probe set.
|
| |
|
| Submission date |
Jun 17, 2011 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Alisha Holloway |
| E-mail(s) |
[email protected]
|
| Organization name |
Gladstone Institutes
|
| Street address |
1650 Owens Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30076 |
Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis |
|
