|
| Status |
Public on Aug 05, 2011 |
| Title |
day 2 epiblast like cells (EpiLCs), biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
day 2 EpiLCs
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: epiblast like cells (EpiLCs)
amplification: --
|
| Growth protocol |
ES cells were maintained with 2i+LIF, feeder free condition. EpiLCs were induced from the ES cells. PGCLCs were induced from day 2 EpiLCs. EpiSCs were derived from epiblast (embryonic day 5.75). For detail, see Hayashi et al., Cell 2011.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Qiagen RNeasy according to manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic One-Cycle Target Labeling Assay)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic Target Hybridization)
|
| Scan protocol |
The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix)
|
| Description |
EA0840_03
|
| Data processing |
CEL data were generated by GeneChip Operating Software and then subjected to the dCHIP software. Data were normalized together with the default settings. The Model-Based Expression Indices (MBEI) were calculated using the PM/MM difference mode with log-2 transformation of signal intensity and truncation of low values to zero.
|
| |
|
| Submission date |
Jun 17, 2011 |
| Last update date |
Nov 28, 2012 |
| Contact name |
Kazuki Kurimoto |
| E-mail(s) |
[email protected]
|
| Organization name |
Nara Medical University
|
| Department |
School of medicine
|
| Lab |
Department of Embryology
|
| Street address |
840 Shijo-Cho, Kashihara
|
| City |
Nara |
| ZIP/Postal code |
634-8521 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE30056 |
Reconstitution of the mouse germ-cell specification pathway in culture by pluripotent stem cells |
|
| Relations |
| Reanalyzed by |
GSM1045429 |
